5'P-RNA: scfAfAgo bound RNA with 5'P - SRA

SRX20566777: 5'P-RNA: scfAfAgo bound RNA with 5'P
1 ILLUMINA (Illumina MiniSeq) run: 3.3M spots, 493.5M bases, 141.3Mb downloads

Design: To obtain AfAgo-bound nucleic acids, E. coli BL21(DE3) was transformed with pBAD_TwinStrep-scfAfAgo plasmid. Cells were grown at 37C in LB medium in the presence of 50g/ml ampicillin until OD600=0.7 was reached. Then, expression was induced by adding 0.2% w/v L-arabinose, and cells were harvested after 4h. Cells were disrupted by incubating 1h at 30C in lysis buffer, containing 20mM Tris-HCl (pH8.0 at 25C), 100mM NaCl, 2mM phenylmethylsulphonyl fluoride, 5mM 2-mercaptoethanol 3mg/ml lysozyme (ThermoFisher cat#89833). The scfAfAgo-NA complex was purified using a StrepTactin column, all buffer solutions contained 100mM NaCl. scfAfAgo-bound RNA was purified using phenol-chloroform isolation as described in Zaremba et al., 2021 (doi: https://doi.org/10.1038/s41564-022-01239-0). Then RNA sample was converted to a DNA library using Small RNA-Seq Library Prep Kit (Lexogen cat#052).

Submitted by: Vilnius University

Study: Archaeoglobus fulgidus Argonaute bound RNA

PRJNA978552 • SRP440679 • All experiments • All runs

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RNA fragments co-purified with the AfAgo protein, scfAfAgo protein, and fAfAgo protein complex from Archaeoglobus fulgidus, examined to determine preferentially bound RNA sequences

Sample: 5'P-RNA: scfAfAgo bound RNA with 5'P

SAMN35557079 • SRS17871296 • All experiments • All runs

Organism: Archaeoglobus fulgidus DSM 4304

Library:

Name: 5p-rna-scfafago

Instrument: Illumina MiniSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 3.3M spots, 493.5M bases, 141.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR24794386	3,267,939	493.5M	141.3Mb	2023-06-01

ID:: 27993777

SRA

Sequence Read Archive

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